Izdelki za oprema za rešitve (3)

RAMOS CARS is the multifunctional multi-channel 3D scanning laser microscope-spectrometer. RAMOS CARS is intended for simultaneous and multifunctional analysis: high-speed imaging by CARS signal; confocal Raman imaging; confocal fluorescence imaging; confocal imaging in the reflected laser radiation; high-contrast imaging in the transmitted laser radiation; as well as visualization of a surface profile by means of the second harmonics generation signal. Microscope RAMOS CARS combines in one system: - CARS scanning microscope - Raman/luminescent scanning confocal microscope - Conventional scanning confocal laser microscope Applications: - Nanobiotechnology: real-time noninvasive analysis of biological samples (cells and components of living cells) with high spatial resolution. - Micro- and nanotechnology investigations of properties of non-biological microstructures: semiconductors, liquid crystals, polymers, pharmaceutical components, micro- and nanoparticles. Spatial XYZ resolution of CARS signals (Objective lens: 60х, NA=1.2, water immersion):< 0.7 μm Spatial resolution of Raman signals:XY< 300 nm, Z< 700 nm Spectral detectable range:CARS signals: 985 – 5000 cm-1, Raman signals: 75 – 6000 cm-1 Spectral resolution of CARS signals:7-8 cm-1 Spectral resolution of Raman signals:0.25 cm-1(grating 75 l/mm Echelle); 0.6 cm-1 (grating 1800 g/mm) Scanning range (fast scanning mode, 60x lens):XY: 225 х 225 μm, Z: 80 μm Control and automation:Fully motorized Optical microscope type:inverted Optical microscope model:Nikon Ti-U Sample stage:motorized - travel range:114 х 75 mm - accuracy (for 1 mm traveling):0.06 μm - XY repeatability:± 1 μm - minimal step:0.02 μm Micro-objective lens:60 х NA-1.2 water immersion, 20 x NA-0.45 Z-scanner type:piezo scanner Z-scanner lens movement range:80 μm Z-scanner minimal step:50 nm Z-scanner repeatability:< 6 nm Illuminator for reflection mode:Halogen lamp 100 W Illuminator for transmission mode:LED Laser input port:motorized triple turret Detector (Raman, E-CARS, fast measurements):Hamamatsu Photosensor module H7844 (with TE cooling) Scanners:Galvano-mirror scanners (XY) Scanning Model:raster high speed and start-stop mode Scanning area:225 μm х 225 μm (objective lens 60Х) Full frame scanning speed:4 s/frame: 1000 х 1000 points Confocal pinhole:motorized confocal pinhole, continuously adjustable from 0 to 1.5 mm Number of registration channels:5

RAMOS E/M series Raman spectrometers are designed on the basis of research-grade optical microscopes allowing the realization of the following light microscopy methods: - Raman measurements - Transmitted light - Reflected light (bright field and dark field illumination) - Confocal microscopy - Fluorescence measurements - Polarization contrast and phase-contrast imaging - Differential interference contrast 3D scanning laser Raman microscopes provide rapid, high sensitivity analysis. The innovative approach to system design of Raman spectrometers ensures extremely high temperature and temporal stability of spectral measurements. All RAMOS E200 system components are fully integrated within an optical microscope providing compactness and mobility of the system. In RAMOS M350, M520, M750 systems external imaging spectrographs are connected via optical fibers. Raman measurements with the RAMOS E/M Series systems can be started in several minutes by turning a system key. Image acquisition mode::3D (XYZ) confocal laser and Raman images Scanning type::XY galvano-mirror scanners, XY motorized stage (option), Z piezo scanner Scanning speed::1000 х 1000 pixels per 3 sec (3 μs/pixel) Spatial resolution::XY: 440 nm, Z: 620 nm (532 nm laser, 100 x , NA = 0.95) Spectral range::50 – 8500 cm-1 (532 nm laser); 50 – 9700 cm-1 (532 nm laser) Excitation source::Build-in 473nm or 532nm, connection of additional external lasers 455nm, 633nm, 785nm is possible Laser beam attenuator::Automated unit with VND filter, continuously changeable from 0.1% to 100% Rayleigh rejection filters::A pair of Edge filters with cut-off starting from 50 cm-1 (for 532 nm laser) Spectrometer configuration::2-channel imaging spectrometer directly coupled to a microscope or External imaging spectrograph Focal length::200 mm, 350 mm, 520 mm or 750 mm Number of gratings::2 gratings, 4 optionally or 4 mounted on motorized turret, more gratings with manual changing Detection system::CCD sensor 2048х122 pixels, with Peltier cooling or CCD sensor 2048х122 with two-stage cooling Options::Heating & cooling stages, fiber optic probe, EM-CCD sensor for ultimate Raman mapping speed Extentions::FLIM option is available. Combination with AFM is possible

Simultaneous / Multifunctional Analysis: - Raman - Luminescence - Laser Reflection & Transmission - Spectral and Polarization measurements The pinhole aperture rejects the residual scattered rays originating from any out-of-focus points on a sample. Spatial resolution less than 500 nm (Z), 200 nm (XY) Spectral resolution ~0.25 cm-1 Wavelength accuracy in spectrum with CCD detector 0.005 nm (1800 l/mm) Specially designed imaging spectrometer incorporates many features that make it ideal for confocal Raman measurements. The image of the pinhole is projected to a multichannel detector without any aberrations. People with little or no experience in Raman spectroscopy can use RAMOS N500. The system is highly modular and fully automated. Up to 5 lasers can be used. The lasers can be switched from one to another with just one click. Motorized control for laser power, beam diameter, polarization orientation, pinhole size, and grating is provided. Microscope type:Upright or inverted Objective lenses:CFI Plan Fluor 4x, 10x, 20x, 40x, 60x, CF Epi Plan APO 100x Stage:Automated, 114x75 mm travel range. Accuracy (1 mm of translation) 0.06 μm. XY repeatability ± 1 μm Z-scanner (objective):Piezo scanner. Translation range 80 μm. Step 50 nm. Repeatability <6 nm Spectral range:325 - 1100 nm Laser radiation delivery:Single, double, triple or penta input port Polarizers (excitation and detection channels):Glan-Taylor prism, 325 - 1000 nm Half-wave plate (λ/2) positioner:Five-position Beam expander:Magnification factor 1-4 Edge filter positioner:Five-position Interference filter positioner:Six-position Optical mechanical unit and microscope coupling:Three- or five- position switch Spatial resolution:XY: <300 nm, Z: 600 nm (532 nm laser, 100x, NA = 0.9) Monochromator focal length:520 mm Monochromator ports:1 input, 2 output Flat field:28 x 10 mm Grating unit:4-position turret Grating choice:150, 300, 600, 1200, 2400, 3600 l/mm, Echelle (75 l/mm) Spectral resolution:0.25 cm-1 for Echelle grating, 0.9 cm-1 for 1800 I/mm grating (wavelength 500 nm) Confocal pinhole:Width 0 - 1.5 mm, step size 0.5 μm Wavelength accuracy with CCD camera:0.005 nm (1800 I/mm grating) Scanning method:Galvano-scanners of XY mirrors Scanning speed:3 sec (1001 x 1001 pixels, min. step 20 nm) Scanning region:150 μm x 150 μm (using 100x objective lens) CCD camera type:Digital CCD camera HS101H Sensor:Back-thinned CCD array 2048 x 122 Pixel size:12 x 12 μm Sensor cooling:Two-stage Peltier cooling with temperature stabilization to -45°C Camera ADC:16 bit Objective positioner:Three-coordinated (XYZ) Laser beam attenuator:VND filter Confocal pinhole:Variable. From 0 to 1.5 mm. Step size 0.5 μm Confocal laser microscope detector:PMT Lasers:325nm (10 mW), 441.6nm (50 mW), 473nm (22/50 nm), 532nm (22/50 mW), 633nm (10 mW), 785nm (100 mW)